• microfluidic encapsulation of cells in alginate particles via an improved internal gelation approach

    جزئیات بیشتر مقاله
    • تاریخ ارائه: 1392/07/24
    • تاریخ انتشار در تی پی بین: 1392/07/24
    • تعداد بازدید: 1099
    • تعداد پرسش و پاسخ ها: 0
    • شماره تماس دبیرخانه رویداد: -
     an improved internal gelation approach is developed to encapsulate single mammalian cells in monodisperse alginate microbeads as small as 26 μm in diameter and at rates of up to 1 khz with high cell viability. the cell damage resulting from contact with calcium carbonate nanoparticles as gelation reagents is eliminated by employing a co-flow microfluidic device, and the cell exposure to low ph is minimized by a chemically balanced off-chip gelation step. these modifications significantly improve the viability of cells encapsulated in gelled alginate particles. two different mammalian cell types are encapsulated with viability of over 84 %. the cells are functional and continue to grow inside the microparticles.

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