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  • the integration of 3-d cell printing and mesoscopic fluorescence molecular tomography of vascular constructs within thick hydrogel scaffolds

    جزئیات بیشتر مقاله
    • تاریخ ارائه: 1391/01/01
    • تاریخ انتشار در تی پی بین: 1391/01/01
    • تعداد بازدید: 771
    • تعداد پرسش و پاسخ ها: 0
    • شماره تماس دبیرخانه رویداد: -
     developing methods that provide adequate vascular perfusion is an important step toward engineering large functional tissues. meanwhile, an imaging modality to assess the three-dimensional (3-d) structures and functions of the vascular channels is lacking for thick matrices (>2∼3 mm). herein, we report on an original approach to construct and image 3-d dynamically perfused vascular structures in thick hydrogel scaffolds. in this work, we integrated a robotic 3-d cell printing technology with a mesoscopic fluorescence molecular tomography imaging system, and demonstrated the capability of the platform to construct perfused collagen scaffolds with endothelial lining and to image both the fluid flow and fluorescent-labeled living endothelial cells at high-frame rates, with high sensitivity and accuracy. these results establish the potential of integrating both 3-d cell printing and fluorescence mesoscopic imaging for functional and molecular studies in complex tissue-engineered tissues.

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