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  • characterization of thauera-dominated hydrogen-oxidizing autotrophic denitrifying microbial communities by using high-throughput sequencing

    جزئیات بیشتر مقاله
    • تاریخ ارائه: 1390/01/01
    • تاریخ انتشار در تی پی بین: 1390/01/01
    • تعداد بازدید: 805
    • تعداد پرسش و پاسخ ها: 0
    • شماره تماس دبیرخانه رویداد: -
     the present study, for the first time, reported a thauera-dominated hydrogen-oxidizing autotrophic denitrifying microbial community enriched from different seed sludges including activated sludge and anaerobic digestion sludge. after 244 days enrichment, nitrogen removal rates reached up to 0.2 mg n/mg vss/d which were comparable to that of the model organism paracoccus denitrificans under the same conditions. furthermore, high-throughput sequencing was applied to characterize and compare the seed sludges and enriched cultures. operational taxonomic units (otu)-based analysis (97% similarity cutoff) of total 280,000 16s rrna gene v6 region sequences from 7 sludge samples (40,000 sequences per sample) revealed that the microbial diversity decreased after the enrichment, indicated by otu numbers drop of 55–60%. thaueraspecies in the class of β-proteobacteria were enriched into the dominant populations with relative abundances of 47–62%, regardless of seed sludge sources.

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