• chitosan–halloysite hybrid-nanotubes: horseradish peroxidase immobilization and applications in phenol removal

    جزئیات بیشتر مقاله
    • تاریخ ارائه: 1392/01/01
    • تاریخ انتشار در تی پی بین: 1392/01/01
    • تعداد بازدید: 581
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     chitosan–halloysite hybrid-nanotubes were synthesized through the assembly of chitosan onto halloysite, a natural nanotubular aluminosilicate. nitrogen adsorption–desorption measurement, fourier-transform infrared spectroscopy, scanning electron microscopy, and transmission electron microscopy analysis were employed to elucidate the structure of the hybrid-nanotubes. the results indicated that the hybrid-nanotubes could form three-dimensional (3d) nanocomposites with hierarchically porous structure. as-prepared structure showed excellent capacity for horseradish peroxidase (hrp) immobilization through cross-linking by glutaraldehyde. the maximum enzyme loading reached as large as 21.5 mg/g, higher than 3.1 mg/g of raw halloysite. after 35 days storage, the immobilized hrp did not undergo any activity loss while the free hrp only retained 27% of its original activity. phenol removal efficiency by the immobilized hrp was also explored. the result showed the immobilized hrp exhibited overall high removal efficiency for phenol from wastewater.

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