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  • co-production of 3-hydroxypropionic acid and 1,3-propanediol by klebseilla pneumoniae expressing aldh under microaerobic conditions

    جزئیات بیشتر مقاله
    • تاریخ ارائه: 1390/01/01
    • تاریخ انتشار در تی پی بین: 1390/01/01
    • تعداد بازدید: 628
    • تعداد پرسش و پاسخ ها: 0
    • شماره تماس دبیرخانه رویداد: -
     fed-batch cultures of klebsiella pneumoniae expressing escherichia coli aldh were performed under microaerobic conditions to investigate the effects on metabolites production. increasing the aeration rate enhanced cell growth and 3-hydroxypropionic acid (3-hp) production, but reduced 1,3-propanediol (1,3-pdo) formation. the recombinant strain k. pneumoniae/puc18kan-aldhec produced 48.9 g/l of 3-hp and 25.3 g/l of 1,3-pdo with an overall yield of 0.66 mol/mol in 28 h at an aeration rate of 1.5 vvm; however, under fully aerobic condition, no 3-hp and 1,3-pdo were produced due to the repression of dha operon. the flux through the reaction catalyzed by glycerol dehydratase and the split ratio of 1,3-pdo were negatively correlated with the aeration rate, even though the 3-hp level showed a positive trend. this study demonstrated that the relative amounts of 3-hp and 1,3-pdo can be controlled by the aeration rate.

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