detection and quantitation of lipid in the microalga tetraselmis subcordiformis (wille) butcher with bodipy 505/515 staining

bodipy 505/515, a lipophilic bright green fluorescent dye was tested for lipid detection in the microalga tetraselmis subcordiformis. a concentration of 0.28 μg ml⁻¹ and staining for 6 min was optimal. lipid bodies stained with bodipy505/515 had a characteristic green fluorescence. their volumes were determined using the sphere volume formula. lipid accumulation under different nitrogen concentrations was analyzed. with an increase in nano₃ concentration from 0 to 240 mg l⁻¹, the maximum algal concentration increased from 8.23 ± 0.62 (×10⁵ cells ml⁻¹) to 1.61 ± 0.13 (×10⁶ cells ml⁻¹), while the maximum volume of intracellular neutral lipid decreased from 9.78 ± 1.77 μm³ cell⁻¹ to 6.00 ± 0.59 μm³ cell⁻¹. a comparison of the lipid contents measured by bodipy 505/515 staining and the gravimetric method showed a positive correlation coefficient ofr² = 0.93. bodipy 505/515 staining is a promising method in lipid quantitation in t. subcordiformis.