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  • removal of water-insoluble sudan dyes by shewanella oneidensis mr-1

    جزئیات بیشتر مقاله
    • تاریخ ارائه: 1390/01/01
    • تاریخ انتشار در تی پی بین: 1390/01/01
    • تعداد بازدید: 847
    • تعداد پرسش و پاسخ ها: 0
    • شماره تماس دبیرخانه رویداد: -
     decolorization of water-insoluble sudan dyes was studied with shewanella oneidensismr-1, which removed 66.8%, 43.4%, 56.0% and 33.7% sudan i–iv in 104 h, respectively and reduced sudan i to aniline and 1-amino-2-naphthol. lactate was identified as the most efficient electron donor for sudan i reduction. improved reduction performance was obtained in the presence of higher lactate or biomass concentration. the correlation between specific reduction rate and initial sudan i concentration could be described with michaelis–menten kinetics (vmax = 1.8 mg sudan i mg cell−1 h−1 andkm = 5.3 mg l−1). the addition of anthraquinone-2-sulfonate stimulated the reduction significantly whereas the presence of 2-hydroxy-1,4-naphthoquinone had little enhancing effect. the main azoreductase activity was found with membrane-bound proteins of mr-1 and no reduction occurred when sudan i was incubated with cell extracts. these data indicated for the first time that shewanella could reduce solid-phase sudan dye particles.

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